DAP-1, a novel protein that interacts with the guanylate kinase-like domains of hDLG and PSD-95

Genes Cells. 1997 Jun;2(6):415-24. doi: 10.1046/j.1365-2443.1997.1310329.x.

Abstract

Background: The human homologue of the Drosophila discs large tumour suppressor protein (hDLG) and closely related proteins such as postsynaptic density protein 95 kDa (PSD-95) are associated with N-methyl-D-aspartate receptors (NMDA-R) and Shaker-type K+ channels, and are thought to be involved in their clustering.

Results: We have identified a protein named DAP-1 that binds to the guanylate kinase-like domains of hDLG and PSD-95. DAP-1 was found to associate with hDLG, PSD-95, NMDA-R and adenomatous polyposis coli protein (APC). Furthermore, we found that DAP-1 is specifically expressed in the brain and colocalizes with PSD-95 and APC in mouse cerebellum. We also found that DAP-1 is colocalized with PSD-95 and NMDA-R at the synapses in cultured rat hippocampal neurons.

Conclusion: Our findings suggest that DAP-1 may play several roles in the molecular organization of synapses and neuronal cell signalling by interacting with hDLG and PSD-95, which in turn are associated with receptors, ion channels and APC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Adenomatous Polyposis Coli Protein
  • Amino Acid Sequence
  • Animals
  • Apoptosis Regulatory Proteins
  • Binding Sites
  • Brain / metabolism
  • Cerebellum / metabolism
  • Cloning, Molecular
  • Cytoskeletal Proteins / immunology
  • Cytoskeletal Proteins / metabolism
  • Discs Large Homolog 1 Protein
  • Disks Large Homolog 4 Protein
  • Guanylate Kinases
  • Hippocampus / metabolism
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Male
  • Membrane Proteins
  • Mice
  • Microscopy, Immunoelectron / methods
  • Molecular Sequence Data
  • Nerve Tissue Proteins / immunology
  • Nerve Tissue Proteins / metabolism*
  • Neurons / metabolism
  • Nucleoside-Phosphate Kinase / metabolism*
  • Precipitin Tests
  • Proteins / genetics*
  • Proteins / immunology
  • Proteins / metabolism*
  • Rabbits
  • Rats
  • Receptors, N-Methyl-D-Aspartate / immunology
  • Receptors, N-Methyl-D-Aspartate / metabolism
  • Repetitive Sequences, Nucleic Acid
  • SAP90-PSD95 Associated Proteins
  • Sequence Homology, Amino Acid

Substances

  • Adaptor Proteins, Signal Transducing
  • Adenomatous Polyposis Coli Protein
  • Apoptosis Regulatory Proteins
  • Cytoskeletal Proteins
  • DAP protein, human
  • DLG1 protein, human
  • DLGAP1 protein, human
  • Discs Large Homolog 1 Protein
  • Disks Large Homolog 4 Protein
  • Dlg1 protein, mouse
  • Dlg1 protein, rat
  • Dlg4 protein, mouse
  • Dlg4 protein, rat
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Proteins
  • Receptors, N-Methyl-D-Aspartate
  • SAP90-PSD95 Associated Proteins
  • postsynaptic density proteins
  • Nucleoside-Phosphate Kinase
  • Guanylate Kinases

Associated data

  • GENBANK/AB000275
  • GENBANK/AB000276
  • GENBANK/AB000277