Abstract
We identified three novel transporters structurally belonging to the organic anion transporting polypeptide (OATP) family in humans. Since previously known rat oatp1 to 3 do not necessarily correspond to the human OATPs in terms of either tissue distribution or function, here we designate the newly identified human OATPs as OATP-B, -D and -E, and we rename the previously known human OATP as OATP-A. OATP-C proved to be identical with the recently reported LST1/OATP-2. Expression profiles of the five OATPs and the prostaglandin transporter PGT (a member of OATP family) in human tissues showed that OATP-C is exclusively localized in liver, OATP-A and PGT are expressed in restricted ranges of tissues, and OATP-B, -D and -E show broad expression profiles. OATP-B, -C, -D and -E exhibited transport activity for [(3)H]estrone-3-sulfate as a common substrate. OATP-C has a high transport activity with broad substrate specificity.
Copyright 2000 Academic Press.
MeSH terms
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Amino Acid Sequence
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Anion Transport Proteins
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Antiporters / chemistry
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Antiporters / genetics
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Biological Transport
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Carrier Proteins / chemistry
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Carrier Proteins / classification
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism*
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Cell Line
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Cloning, Molecular
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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Dinoprostone / metabolism
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Estradiol / analogs & derivatives
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Estradiol / metabolism
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Estrone / analogs & derivatives
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Estrone / metabolism
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Gene Expression Profiling
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Humans
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Molecular Sequence Data
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Multigene Family / genetics*
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Organ Specificity
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Organic Anion Transporters
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Penicillin G / metabolism
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Phylogeny
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Physical Chromosome Mapping
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Polymorphism, Single Nucleotide / genetics
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Sequence Alignment
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Substrate Specificity
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Transfection
Substances
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Anion Transport Proteins
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Antiporters
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Carrier Proteins
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DNA-Binding Proteins
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Organic Anion Transporters
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RNA, Messenger
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SLCO2A1 protein, human
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Slco2a1 protein, rat
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estradiol-17 beta-glucuronide
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Estrone
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Estradiol
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Dinoprostone
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Penicillin G
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estrone sulfate
Associated data
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GENBANK/AB026256
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GENBANK/AB026257
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GENBANK/AB031050
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GENBANK/AB031051