Abstract
Using a conventional two-hybrid technique with MAWD as bait protein, a novel full-length cDNA was isolated and sequenced from a human liver cDNA library. This cDNA consists of 2,575 base pairs and has a predicted open reading frame encoding 255 amino acids. Overall, it is similar to the catalytic enzyme PHZF, catalyzing the hydroxylation of phenazine-1-carboxylic acid to 2-hydroxyphenazine-1-carboxylic acid. Polymerase chain reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 10q21.1 near the marker D10S210.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Chromosomes, Human, Pair 10 / genetics
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Cloning, Molecular*
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DNA, Complementary
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Humans
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Liver / enzymology
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Mixed Function Oxygenases / analysis
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Mixed Function Oxygenases / genetics*
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Mixed Function Oxygenases / metabolism
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Molecular Sequence Data
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Neoplasm Proteins / metabolism
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Organ Specificity
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Phenazines / metabolism
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Physical Chromosome Mapping
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Proteins
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RNA-Binding Proteins
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Radiation Hybrid Mapping
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Sequence Analysis, DNA*
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Sequence Homology
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Two-Hybrid System Techniques
Substances
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DNA, Complementary
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Neoplasm Proteins
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PBLD protein, human
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Phenazines
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Proteins
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RNA-Binding Proteins
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STRAP protein, human
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1-phenazinecarboxylic acid
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Mixed Function Oxygenases