Cloning and sequencing of a novel human gene which encodes a putative hydroxylase

J Hum Genet. 2001;46(5):289-92. doi: 10.1007/s100380170081.

Abstract

Using a conventional two-hybrid technique with MAWD as bait protein, a novel full-length cDNA was isolated and sequenced from a human liver cDNA library. This cDNA consists of 2,575 base pairs and has a predicted open reading frame encoding 255 amino acids. Overall, it is similar to the catalytic enzyme PHZF, catalyzing the hydroxylation of phenazine-1-carboxylic acid to 2-hydroxyphenazine-1-carboxylic acid. Polymerase chain reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 10q21.1 near the marker D10S210.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosomes, Human, Pair 10 / genetics
  • Cloning, Molecular*
  • DNA, Complementary
  • Humans
  • Liver / enzymology
  • Mixed Function Oxygenases / analysis
  • Mixed Function Oxygenases / genetics*
  • Mixed Function Oxygenases / metabolism
  • Molecular Sequence Data
  • Neoplasm Proteins / metabolism
  • Organ Specificity
  • Phenazines / metabolism
  • Physical Chromosome Mapping
  • Proteins
  • RNA-Binding Proteins
  • Radiation Hybrid Mapping
  • Sequence Analysis, DNA*
  • Sequence Homology
  • Two-Hybrid System Techniques

Substances

  • DNA, Complementary
  • Neoplasm Proteins
  • PBLD protein, human
  • Phenazines
  • Proteins
  • RNA-Binding Proteins
  • STRAP protein, human
  • 1-phenazinecarboxylic acid
  • Mixed Function Oxygenases

Associated data

  • GENBANK/AB049758