Complete genome sequence of BS49 and draft genome sequence of BS34A, Bacillus subtilis strains carrying Tn916

Hilary P Browne; Seyed Yahya Anvar; Jeroen Frank; Trevor D Lawley; Adam P Roberts; Wiep Klaas Smits

doi:10.1093/femsle/fnu050

Complete genome sequence of BS49 and draft genome sequence of BS34A, Bacillus subtilis strains carrying Tn916

FEMS Microbiol Lett. 2015 Jan;362(3):1-4. doi: 10.1093/femsle/fnu050. Epub 2014 Dec 21.

Authors

Hilary P Browne¹, Seyed Yahya Anvar², Jeroen Frank², Trevor D Lawley¹, Adam P Roberts³, Wiep Klaas Smits⁴

Affiliations

¹ Host-Microbiota Interactions Laboratory, Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK.
² Leiden Genome Technology Center, Human and Clinical Genetics, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands.
³ Department of Microbial Diseases, UCL Eastman Dental Institute, WC1X 8 LD London, UK.
⁴ Department of Medical Microbiology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands w.k.smits@lumc.nl.

PMID: 25673660
DOI: 10.1093/femsle/fnu050

Abstract

Bacillus subtilis strains BS49 and BS34A, both derived from a common ancestor, carry one or more copies of Tn916, an extremely common mobile genetic element capable of transfer to and from a broad range of microorganisms. Here, we report the complete genome sequence of BS49 and the draft genome sequence of BS34A, which have repeatedly been used as donors to transfer Tn916, Tn916 derivatives or oriTTn916-containing plasmids to clinically important pathogens.

Keywords: conjugation; conjugative transposon; donor; mobile element.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / genetics*
Base Sequence
Conjugation, Genetic
DNA Transposable Elements*
Genome, Bacterial*
Plasmids
Sequence Analysis, DNA

Substances

DNA Transposable Elements