Distinct differentiation of closely related species of Bacillus subtilis group with industrial importance

Kumaraswamy Jeyaram; Wahengbam Romi; Thangjam Anand Singh; Gbenga Adedeji Adewumi; Khundrakpam Basanti; Folarin Anthony Oguntoyinbo

doi:10.1016/j.mimet.2011.08.011

Distinct differentiation of closely related species of Bacillus subtilis group with industrial importance

J Microbiol Methods. 2011 Nov;87(2):161-4. doi: 10.1016/j.mimet.2011.08.011. Epub 2011 Aug 26.

Authors

Kumaraswamy Jeyaram¹, Wahengbam Romi, Thangjam Anand Singh, Gbenga Adedeji Adewumi, Khundrakpam Basanti, Folarin Anthony Oguntoyinbo

Affiliation

¹ Microbial Resources Division, Institute of Bioresources and Sustainable Development, Takyelpat Institutional Area, Imphal-795001, Manipur, India. jeyaram.ibsd@nic.in

PMID: 21889958
DOI: 10.1016/j.mimet.2011.08.011

Abstract

PCR amplification of 16S rRNA gene by universal primers followed by restriction fragment length polymorphism analysis using RsaI, CfoI and HinfI endonucleases, distinctly differentiated closely related Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus from Bacillus subtilis sensu stricto. This simple, economical, rapid and reliable protocol could be an alternative to misleading phenotype-based grouping of these closely related species.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacillus / classification
Bacillus / genetics
Bacillus / isolation & purification*
Bacillus subtilis / classification
Bacillus subtilis / genetics
Bacillus subtilis / isolation & purification
Bacterial Typing Techniques / methods*
Base Sequence
DNA Primers / genetics
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Industrial Microbiology*
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S / genetics

Substances

DNA Primers
DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S