Abstract
An atypical urease-negative mutant of Actinobacillus pleuropneumoniae serovar 2 was isolated in Japan. Nucleotide sequence analysis of the urease gene cluster revealed that the insertion of a short DNA sequence into the cbiM gene was responsible for the urease-negative activity of the mutant. Veterinary diagnostic laboratories should be watchful for the presence of aberrant urease-negative A. pleuropneumoniae isolates.
Keywords:
Molecular characterization; urease-negative Actinobacillus pleuropneumoniae mutant.
MeSH terms
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Actinobacillus Infections / diagnosis
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Actinobacillus Infections / epidemiology
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Actinobacillus Infections / microbiology
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Actinobacillus Infections / veterinary*
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Actinobacillus pleuropneumoniae / enzymology
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Actinobacillus pleuropneumoniae / genetics
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Actinobacillus pleuropneumoniae / isolation & purification*
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Animals
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Base Sequence
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Japan
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Pleuropneumonia / diagnosis
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Pleuropneumonia / epidemiology
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Pleuropneumonia / veterinary*
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Serogroup
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Swine
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Swine Diseases / diagnosis
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Swine Diseases / epidemiology
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Swine Diseases / microbiology*
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Urease / metabolism*