Abstract
To evaluate the role of uridylyl-transferase, the Sinorhizobium meliloti glnD gene was isolated by heterologous complementation in Azotobacter vinelandii. The glnD gene is cotranscribed with a gene homologous to Salmonella mviN. glnD1::Omega or mviN1::Omega mutants could not be isolated by a powerful sucrose counterselection procedure unless a complementing cosmid was provided, indicating that glnD and mviN are members of an indispensable operon in S. meliloti.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Cloning, Molecular
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Conjugation, Genetic
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Genes, Bacterial
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Genes, Essential
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Genetic Complementation Test
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Molecular Sequence Data
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Mutagenesis, Insertional
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Nucleotidyltransferases / genetics*
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Nucleotidyltransferases / metabolism
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Operon*
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PII Nitrogen Regulatory Proteins
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Plasmids
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Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sinorhizobium meliloti / genetics
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Sinorhizobium meliloti / metabolism*
Substances
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Bacterial Proteins
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PII Nitrogen Regulatory Proteins
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Nucleotidyltransferases
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regulatory protein uridylyltransferase