Characterization of a novel coronavirus associated with severe acute respiratory syndrome

Paul A Rota; M Steven Oberste; Stephan S Monroe; W Allan Nix; Ray Campagnoli; Joseph P Icenogle; Silvia Peñaranda; Bettina Bankamp; Kaija Maher; Min-Hsin Chen; Suxiong Tong; Azaibi Tamin; Luis Lowe; Michael Frace; Joseph L DeRisi; Qi Chen; David Wang; Dean D Erdman; Teresa C T Peret; Cara Burns; Thomas G Ksiazek; Pierre E Rollin; Anthony Sanchez; Stephanie Liffick; Brian Holloway; Josef Limor; Karen McCaustland; Melissa Olsen-Rasmussen; Ron Fouchier; Stephan Günther; Albert D M E Osterhaus; Christian Drosten; Mark A Pallansch; Larry J Anderson; William J Bellini

doi:10.1126/science.1085952

Characterization of a novel coronavirus associated with severe acute respiratory syndrome

Science. 2003 May 30;300(5624):1394-9. doi: 10.1126/science.1085952. Epub 2003 May 1.

Affiliation

¹ National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. prota@cdc.gov

PMID: 12730500
DOI: 10.1126/science.1085952

Abstract

In March 2003, a novel coronavirus (SARS-CoV) was discovered in association with cases of severe acute respiratory syndrome (SARS). The sequence of the complete genome of SARS-CoV was determined, and the initial characterization of the viral genome is presented in this report. The genome of SARS-CoV is 29,727 nucleotides in length and has 11 open reading frames, and its genome organization is similar to that of other coronaviruses. Phylogenetic analyses and sequence comparisons showed that SARS-CoV is not closely related to any of the previously characterized coronaviruses.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acid Sequence
Conserved Sequence
Coronavirus / classification
Coronavirus / genetics
Coronavirus M Proteins
Coronavirus Nucleocapsid Proteins
DNA, Complementary
Endopeptidases / chemistry
Endopeptidases / genetics
Genome, Viral*
Humans
Membrane Glycoproteins / chemistry
Membrane Glycoproteins / genetics
Molecular Sequence Data
Nucleocapsid Proteins / chemistry
Nucleocapsid Proteins / genetics
Open Reading Frames
Phylogeny
Polyproteins / chemistry
Polyproteins / genetics
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Viral / genetics*
RNA-Dependent RNA Polymerase / chemistry
RNA-Dependent RNA Polymerase / genetics
Regulatory Sequences, Nucleic Acid
Sequence Analysis, DNA
Severe Acute Respiratory Syndrome / virology
Severe acute respiratory syndrome-related coronavirus / chemistry
Severe acute respiratory syndrome-related coronavirus / classification
Severe acute respiratory syndrome-related coronavirus / genetics*
Severe acute respiratory syndrome-related coronavirus / isolation & purification
Spike Glycoprotein, Coronavirus
Transcription, Genetic
Viral Envelope Proteins / chemistry
Viral Envelope Proteins / genetics
Viral Matrix Proteins / chemistry
Viral Matrix Proteins / genetics
Viral Proteins / chemistry
Viral Proteins / genetics*

Substances

Coronavirus M Proteins
Coronavirus Nucleocapsid Proteins
DNA, Complementary
M protein, SARS-CoV
Membrane Glycoproteins
Nucleocapsid Proteins
Polyproteins
RNA, Messenger
RNA, Viral
Spike Glycoprotein, Coronavirus
Viral Envelope Proteins
Viral Matrix Proteins
Viral Proteins
RNA-Dependent RNA Polymerase
Endopeptidases