Cloning and sequence analysis of a novel member of the single-stranded DNA binding protein family

Biochim Biophys Acta. 1995 Mar 14;1261(1):143-6. doi: 10.1016/0167-4781(95)00005-2.

Abstract

A single-stranded DNA binding protein (SSB) was isolated which has unusual amino acid residues at sites previously shown to be highly conserved and critical for DNA binding. Sequence analysis suggested that these residues are characteristic of a novel class of SSBs from Gram-positive bacteria.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins / chemistry
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • DNA, Single-Stranded / metabolism
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / classification
  • DNA-Binding Proteins / genetics*
  • Genes
  • Gram-Positive Bacteria / genetics
  • Molecular Sequence Data
  • Rats / genetics*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity

Substances

  • Bacterial Proteins
  • DNA, Single-Stranded
  • DNA-Binding Proteins

Associated data

  • GENBANK/U12515