The complete mitochondrial genome was sequenced from the freshwater cyprinid fish, Acheilognathus barbatus. The mitochondrial genome sequence was 16,770 bp in size, and the gene order and contents were identical with the congeneric species A. macropterus, A. typus and A. yamatsutae. Six genes (COII, ND2, COIII, ND3, ND4 and Cytb) had an incomplete stop codon as shown in other cyprinid fishes. Base composition of the genome is A (29.1%), T (26.9%), C (26.6%) and G (17.4%) with an A + T rich feature (56%) as that of other vertebrate mitochondrial genomes.
Keywords: Acheilognathus barbatus; complete mitochondrial genome; cyprinidae.