Abstract
The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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DNA, Recombinant / genetics
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Escherichia coli / genetics
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Genetic Vectors*
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Molecular Sequence Data
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Plants / microbiology*
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Plasmids / genetics
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Pseudomonas fluorescens / genetics*
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Replicon / genetics*
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Sequence Homology, Amino Acid
Associated data
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GENBANK/AF118810
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GENBANK/AF118811
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GENBANK/AF118812
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GENBANK/AF133828
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GENBANK/AF133829
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GENBANK/AF133830
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GENBANK/AF133831
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GENBANK/U10456
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GENBANK/U10457
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GENBANK/U10458
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GENBANK/U10459
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GENBANK/U10460
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GENBANK/U10461
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GENBANK/U10462
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GENBANK/U10463
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GENBANK/U10487
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GENBANK/U10488
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GENBANK/U10489
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GENBANK/U10490
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GENBANK/U10491