Embryonic lung fibroblasts (HLF) infected with control lentivirus, or lentivirus expressing MRG15 and MRGX small hairpin RNAs from the H1 polIII promoter. The MRG15 shRNA induces an interferon response that is unrelated to silencing of MRG15.
Bridge AJ, Pebernard S, Ducraux A, Nicoulaz AL et al. Induction of an interferon response by RNAi vectors in mammalian cells. Nat Genet 2003 Jul;34(3):263-4. PMID: 12796781