Arhgef2 Rho/Rac guanine nucleotide exchange factor 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Arhgef2provided by RGD
Official Full Name: Rho/Rac guanine nucleotide exchange factor 2provided by RGD
Primary source: RGD:1304659
See related: EnsemblRapid:ENSRNOG00000020027 AllianceGenome:RGD:1304659
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Predicted to enable guanyl-nucleotide exchange factor activity; microtubule binding activity; and small GTPase binding activity. Predicted to be involved in several processes, including cytoskeleton organization; positive regulation of macromolecule metabolic process; and regulation of signal transduction. Predicted to act upstream of or within negative regulation of podosome assembly. Is active in glutamatergic synapse and postsynaptic density, intracellular component. Human ortholog(s) of this gene implicated in neurodevelopmental disorder with midbrain and hindbrain malformations. Orthologous to human ARHGEF2 (Rho/Rac guanine nucleotide exchange factor 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Lung (RPKM 170.0), Spleen (RPKM 135.9) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 2q34

Exon count:: 34

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	2	NC_086020.1 (176358909..176416178)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	2	NC_051337.1 (174061126..174118355)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	2	NC_005101.4 (187964100..188022847)

Chromosome 2 - NC_086020.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results indicated that Tau produced strong plasma membrane blebbing and F-Actin cytoskeleton remodeling by indirect activation of the GEF-H1-RhoA-ROCK pathway. All of these actions were initiated by abnormal, induced microtubule bundling and cytoplasmic release of GEF-H1.
Title: Expression of Tau Produces Aberrant Plasma Membrane Blebbing in Glial Cells Through RhoA-ROCK-Dependent F-Actin Remodeling.
regulation of c-Src trafficking requires both microtubules and actin polymerization, and GEF-H1 coordinates c-Src trafficking, acting as a molecular switch between these two mechanisms
Title: Microtubule and Actin Interplay Drive Intracellular c-Src Trafficking.
upregulation of GEF-H1 activity may contribute to excitotoxicity, whereas the late upregulation of GEF-H1 protein may promote neuroplasticity after brain ischemia.
Title: Upregulation of the GEF-H1 pathway after transient cerebral ischemia.
GEF-H1 activates RhoA in response to RalA GTPase, which in turn regulates the localization and the assembly of exocyst components and exocytosis.
Title: GEF-H1: orchestrating the interplay between cytoskeleton and vesicle trafficking.
complement-induced ERK-dependent GEF-H1 activation as the upstream mechanism of RhoA stimulation, and this pathway has a protective role against cell death.
Title: Role of guanine nucleotide exchange factor-H1 in complement-mediated RhoA activation in glomerular epithelial cells.
GEF-H1 is significantly activated in brain during the post-traumatic brain injury phase.
Title: Changes in the GEF-H1 pathways after traumatic brain injury.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH134330 (e-PCR)
- UniSTS:217612

RH124802 (e-PCR)
- UniSTS:161624

RH141382 (e-PCR)
- UniSTS:223378

D2Rat42 (e-PCR)
- UniSTS:118698

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
enables guanyl-nucleotide exchange factor activity	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	ISO Inferred from Sequence Orthology more info
enables microtubule binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within actin filament organization	ISO Inferred from Sequence Orthology more info
involved_in actin filament organization	ISO Inferred from Sequence Orthology more info
involved_in actin filament organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in asymmetric neuroblast division	IEA Inferred from Electronic Annotation more info
involved_in asymmetric neuroblast division	ISO Inferred from Sequence Orthology more info
involved_in asymmetric neuroblast division	ISS Inferred from Sequence or Structural Similarity more info
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in cell morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell morphogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in cell morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in cell morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular hyperosmotic response	ISO Inferred from Sequence Orthology more info
involved_in cellular response to muramyl dipeptide	IEA Inferred from Electronic Annotation more info
involved_in cellular response to muramyl dipeptide	ISO Inferred from Sequence Orthology more info
involved_in cellular response to muramyl dipeptide	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to tumor necrosis factor	ISO Inferred from Sequence Orthology more info
involved_in establishment of mitotic spindle orientation	ISO Inferred from Sequence Orthology more info
involved_in innate immune response	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of microtubule depolymerization	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of microtubule depolymerization	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of microtubule depolymerization	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of necroptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neurogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of podosome assembly	ISO Inferred from Sequence Orthology more info
involved_in nervous system development	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of Rac protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of epidermal growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-6 production	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of interleukin-6 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-6 production	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of metalloendopeptidase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of neuron differentiation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of neuron differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of neuron migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of p38MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of peptidyl-tyrosine phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of peptidyl-tyrosine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of peptidyl-tyrosine phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of tumor necrosis factor production	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of tumor necrosis factor production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of tumor necrosis factor production	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of wound healing	ISO Inferred from Sequence Orthology more info
involved_in regulation of Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of Rho protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in tumor necrosis factor-mediated signaling pathway	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in bicellular tight junction	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	ISS Inferred from Sequence or Structural Similarity more info
located_in dendritic shaft	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in microtubule	ISO Inferred from Sequence Orthology more info
located_in microtubule	ISS Inferred from Sequence or Structural Similarity more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in podosome	ISO Inferred from Sequence Orthology more info
located_in postsynaptic density	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density, intracellular component	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density, intracellular component	IMP Inferred from Mutant Phenotype more info	PubMed
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISS Inferred from Sequence or Structural Similarity more info
is_active_in ruffle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in ruffle membrane	IEA Inferred from Electronic Annotation more info
located_in ruffle membrane	ISO Inferred from Sequence Orthology more info
located_in ruffle membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in spindle	IEA Inferred from Electronic Annotation more info
located_in vesicle	ISO Inferred from Sequence Orthology more info
located_in vesicle	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: rho guanine nucleotide exchange factor 2

Names: GEF-H1; guanine nucleotide exchange factor H1; rho/rac guanine nucleotide exchange factor (GEF) 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001012079.2 → NP_001012079.1 rho guanine nucleotide exchange factor 2

See identical proteins and their annotated locations for NP_001012079.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000002

UniProtKB/Swiss-Prot

Q5FVC2

UniProtKB/TrEMBL

A6J694

Related

ENSRNOP00000027182.5, ENSRNOT00000027182.9

Conserved Domains (4) summary

cd00029
Location:40 → 85

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00160
Location:237 → 431

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13393
Location:471 → 586

PH_ARHGEF2; Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain

pfam00169
Location:474 → 572

PH; PH domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086020.1 Reference GRCr8

Range

176358909..176416178

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006232643.5 → XP_006232705.1 rho guanine nucleotide exchange factor 2 isoform X1

See identical proteins and their annotated locations for XP_006232705.1

UniProtKB/TrEMBL

A0A0G2K0V4, A0A1B0GWX8

Related

ENSRNOP00000076068.2, ENSRNOT00000093033.3

Conserved Domains (4) summary

cd00160
Location:432 → 626

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

TIGR02168
Location:898 → 1057

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

cd13393
Location:666 → 781

PH_ARHGEF2; Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain

cd20877
Location:230 → 290

C1_ARHGEF2; protein kinase C conserved region 1 (C1 domain) found in Rho guanine nucleotide exchange factor 2 (ARHGEF2) and similar proteins
XM_017590882.3 → XP_017446371.1 rho guanine nucleotide exchange factor 2 isoform X2

UniProtKB/TrEMBL

A0A1B0GWX8

Conserved Domains (4) summary

cd00029
Location:235 → 280

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00160
Location:430 → 624

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13393
Location:664 → 779

PH_ARHGEF2; Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain

pfam00169
Location:667 → 765

PH; PH domain
XM_039102312.2 → XP_038958240.1 rho guanine nucleotide exchange factor 2 isoform X1

UniProtKB/TrEMBL

A0A0G2K0V4, A0A1B0GWX8

Related

ENSRNOP00000071595.1, ENSRNOT00000081531.3

Conserved Domains (4) summary

cd00160
Location:432 → 626

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

TIGR02168
Location:898 → 1057

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

cd13393
Location:666 → 781

PH_ARHGEF2; Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain

cd20877
Location:230 → 290

C1_ARHGEF2; protein kinase C conserved region 1 (C1 domain) found in Rho guanine nucleotide exchange factor 2 (ARHGEF2) and similar proteins
XM_006232646.5 → XP_006232708.1 rho guanine nucleotide exchange factor 2 isoform X3

UniProtKB/TrEMBL

A0A8I6AK99, A6J694

Related

ENSRNOP00000094178.1, ENSRNOT00000105386.2

Conserved Domains (4) summary

cd00029
Location:173 → 218

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00160
Location:370 → 564

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13393
Location:604 → 719

PH_ARHGEF2; Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain

pfam00169
Location:607 → 705

PH; PH domain
XM_063281840.1 → XP_063137910.1 rho guanine nucleotide exchange factor 2 isoform X4
XM_008761152.4 → XP_008759374.1 rho guanine nucleotide exchange factor 2 isoform X8

UniProtKB/TrEMBL

A0A1B0GWY5, A6J694

Conserved Domains (4) summary

cd00029
Location:25 → 70

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00160
Location:222 → 416

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13393
Location:456 → 571

PH_ARHGEF2; Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain

pfam00169
Location:459 → 557

PH; PH domain
XM_063281842.1 → XP_063137912.1 rho guanine nucleotide exchange factor 2 isoform X6
XM_006232652.5 → XP_006232714.1 rho guanine nucleotide exchange factor 2 isoform X9

See identical proteins and their annotated locations for XP_006232714.1

UniProtKB/TrEMBL

A6J694

Conserved Domains (4) summary

cd00029
Location:13 → 58

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00160
Location:210 → 404

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13393
Location:444 → 559

PH_ARHGEF2; Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain

pfam00169
Location:447 → 545

PH; PH domain
XM_063281841.1 → XP_063137911.1 rho guanine nucleotide exchange factor 2 isoform X5

Related

ENSRNOP00000095755.2, ENSRNOT00000099415.2
XM_006232651.5 → XP_006232713.1 rho guanine nucleotide exchange factor 2 isoform X7

UniProtKB/TrEMBL

A0A8I6AFM5, A6J694

Related

ENSRNOP00000091462.1, ENSRNOT00000119126.2

Conserved Domains (4) summary

cd00029
Location:32 → 77

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd00160
Location:229 → 423

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13393
Location:463 → 578

PH_ARHGEF2; Rho guanine nucleotide exchange factor 2 Pleckstrin homology (PH) domain

pfam00169
Location:466 → 564

PH; PH domain