SLA2 Sla2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SLA2
Official Full Name: Sla2p
Primary source: SGD:S000005187
Locus tag: YNL243W
See related: AllianceGenome:SGD:S000005187; FungiDB:YNL243W; VEuPathDB:YNL243W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: END4; MOP2
Summary: Predicted to enable actin filament binding activity; anion binding activity; and clathrin binding activity. Involved in actin cytoskeleton organization; endocytosis; and negative regulation of Arp2/3 complex-mediated actin nucleation. Located in actin cortical patch. Part of incipient cellular bud site. Orthologous to human HIP1 (huntingtin interacting protein 1) and HIP1R (huntingtin interacting protein 1 related). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SLA2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (188051..190957)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis.
Title: Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis.
Elevated PM tension, upon TORC2 inhibition, impinges on endocytosis at two different levels by (1) severing the bonds between the PM adaptor proteins Sla2 and Ent1 and the actin cytoskeleton and (2) hindering recruitment of Rvs167, an N-BAR-containing protein important for vesicle fission to endocytosis sites
Title: TORC2 controls endocytosis through plasma membrane tension.
analysis of the molecular mechanism of plasma membrane-actin cytoskeleton coupling mediated by cooperative action of epsin Ent1 and the HIP1R homolog Sla2 in yeast Saccharomyces cerevisiae
Title: Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis.
CLC directs endocytic progression by pruning the Sla2-actin attachments in the clathrin lattice, providing direction for membrane internalization
Title: Clathrin light chain directs endocytosis by influencing the binding of the yeast Hip1R homologue, Sla2, to F-actin.
growth defects in cells lacking SLA2 are associated with a cell cycle delay that is influenced by Swe1, a morphogenesis checkpoint kinase
Title: SLA2 mutations cause SWE1-mediated cell cycle phenotypes in Candida albicans and Saccharomyces cerevisiae.
Two recessive mutations, act1-301 in the actin gene and sla2-82 in a gene involved in cortical actin patch assembly, were identified.
Title: Identification of novel mutations in ACT1 and SLA2 that suppress the actin-cable-overproducing phenotype caused by overexpression of a dominant active form of Bni1p in Saccharomyces cerevisiae.
We propose that LC binding to Sla2p positively regulates Sla2p for efficient endocytic vesicle formation.
Title: Novel function of clathrin light chain in promoting endocytic vesicle formation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables clathrin adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables clathrin binding	IEA Inferred from Electronic Annotation more info
enables clathrin light chain binding	IBA Inferred from Biological aspect of Ancestor more info
enables molecular_function	ND No biological Data available more info
enables phosphatidylinositol-3,4-bisphosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol-3,5-bisphosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables phospholipid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin cortical patch assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in clathrin coat assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of Arp2/3 complex-mediated actin nucleation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in actin cortical patch	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
located_in actin cortical patch	IEA Inferred from Electronic Annotation more info
located_in cell cortex	IEA Inferred from Electronic Annotation more info
located_in cellular bud neck	HDA more info	PubMed
located_in cellular bud tip	IEA Inferred from Electronic Annotation more info
is_active_in clathrin-coated vesicle	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cortical actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	HDA more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Sla2p

NP_014156.2

Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

188051..190957

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183081.2 → NP_014156.2 TPA: Sla2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014156.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0V0, P33338, Q02434

UniProtKB/TrEMBL

B3LP82, C7GPB8, C8ZFZ3, N1P530

Conserved Domains (3) summary

smart00307
Location:763 → 965

ILWEQ; I/LWEQ domain

COG1196
Location:420 → 546

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

pfam07651
Location:7 → 269

ANTH; ANTH domain