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    tsr methyl-accepting chemotaxis protein Tsr [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948884, updated on 19-Sep-2024

    Summary

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    Official Symbol
    tsr
    Official Full Name
    methyl-accepting chemotaxis protein Tsr
    Primary source
    ECOCYC:EG11034
    Locus tag
    b4355
    See related
    ASAP:ABE-0014282
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    cheD; ECK4345
    Summary
    Tsr also senses energy levels and is involved in aerotaxis, redox taxis and glycerol repellent taxis. [More information is available at EcoGene: EG11034]. Tsr is a serine chemoreceptor in Escherichia coli and binds directly to the MCP periplasmic domain. [More information is available at EcoCyc: EG11034].
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    Genomic context

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    Sequence:
    NC_000913.3 (4591657..4593312)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF04328 family protein YjiX Neighboring gene pyruvate:H(+) symporter Neighboring gene galactonate:H(+) symporter Neighboring gene putative DNA-binding transcriptional regulator LgoR

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Role of a small cytoplasmic domain in the establishment of serine chemoreceptor membrane topology. Kimbrough TG, et al. J Bacteriol, 1994 Nov. PMID 7961482, Free PMC Article
    2. Structure of the serine chemoreceptor in Escherichia coli. Boyd A, et al. Nature, 1983 Feb 17-23. PMID 6402709
    3. Genetic and biochemical properties of Escherichia coli mutants with defects in serine chemotaxis. Hedblom ML, et al. J Bacteriol, 1980 Dec. PMID 6777365, Free PMC Article
    4. Novel mutations affecting a signaling component for chemotaxis of Escherichia coli. Parkinson JS. J Bacteriol, 1980 Jun. PMID 6991496, Free PMC Article
    5. Universal architecture of bacterial chemoreceptor arrays. Briegel A, et al. Proc Natl Acad Sci U S A, 2009 Oct 6. PMID 19805102, Free PMC Article

    See all (119) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural signatures of Escherichia coli chemoreceptor signaling states revealed by cellular crosslinking.
      Title: Structural signatures of Escherichia coli chemoreceptor signaling states revealed by cellular crosslinking.
    2. The authors show that individual Tsr dimers within a trimer exhibit asymmetric flexibilities that are a function of the signaling state, highlighting the effect of their different protein interactions at the receptor tips.
      Title: In Situ Conformational Changes of the Escherichia coli Serine Chemoreceptor in Different Signaling States.
    3. Blue light is a universal signal for Escherichia coli chemoreceptors.
      Title: Blue Light Is a Universal Signal for Escherichia coli Chemoreceptors.
    4. this study shows that Tsr interacts with IL-8 provoking E. coli transmigration across human lung epithelial cells
      Title: Tsr Chemoreceptor Interacts With IL-8 Provoking E. coli Transmigration Across Human Lung Epithelial Cells.
    5. results indicate that, rather than being essential for proper receptor-receptor interaction, the "glycine hinge" residues are involved in the ability of the receptor to switch between different signaling states. Mainly, the C-helix residue G439 has a key role in shifting the equilibrium toward a kinase-activating conformation.
      Title: Mutational Replacements at the "Glycine Hinge" of the Escherichia coli Chemoreceptor Tsr Support a Signaling Role for the C-Helix Residue.
    6. These results indicate that the E402 and R404 residues of Tsr play their most critical signaling roles at their inner locations near the trimer axis where they likely participate in stabilizing the trimer-of-dimer packing and the kinase-ON state of core signaling complexes.
      Title: Signaling Consequences of Structural Lesions that Alter the Stability of Chemoreceptor Trimers of Dimers.
    7. The authors suggest that the Tsr control cable transmits input signals to a four-helix HAMP bundle by modulating the intensity of structural clashes between out-of-register transmembrane helix and AS1 helix of HAMP.
      Title: Evidence for a Helix-Clutch Mechanism of Transmembrane Signaling in a Bacterial Chemoreceptor.
    8. Only strains that produce TynA and FeaB converting norepinephrine to 3,4-dihydroxymandelic acid xhibited chemotaxis toward norepinephrine and 3,4-dihydroxymandelic acid is sensed by the serine chemoreceptor Tsr.
      Title: Chemotaxis of Escherichia coli to norepinephrine (NE) requires conversion of NE to 3,4-dihydroxymandelic acid.
    9. Phe396 governs conformational changes of tsr.
      Title: A phenylalanine rotameric switch for signal-state control in bacterial chemoreceptors.
    10. Co-overexpression of Tsr, CheA, and CheW yields hexagonal Arrays.
      Title: New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography.
    Submit: New GeneRIF Correction See all GeneRIFs (31)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transmembrane signaling receptor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transmembrane signaling receptor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in cell motility IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to amino acid stimulus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in chemotaxis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in chemotaxis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chemotaxis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in detection of chemical stimulus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in receptor clustering IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of bacterial-type flagellum-dependent cell motility IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of chemotaxis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of protein histidine kinase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in signal complex assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in signal transduction IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in signal transduction IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of methyl accepting chemotaxis protein complex IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    methyl-accepting chemotaxis protein Tsr

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4591657..4593312
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418775.1 methyl-accepting chemotaxis protein Tsr [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418775.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7EFF2
      Conserved Domains (1) summary
      PRK15041
      Location:1 → 551
      PRK15041; methyl-accepting chemotaxis protein
    Nucleotide Protein
    Heading Accession and Version

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