Bco1 beta-carotene oxygenase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Bco1provided by MGI
Official Full Name: beta-carotene oxygenase 1provided by MGI
Primary source: MGI:MGI:1926923
See related: Ensembl:ENSMUSG00000031845 AllianceGenome:MGI:1926923
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Bcdo; CMO1; Cmoi; Bcdo1; Bcmo1; beta-CD; betaCMOOX
Summary: Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. Two alternatively spliced variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
Expression: Biased expression in testis adult (RPKM 9.3), liver E18 (RPKM 2.0) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 E1; 8 63.61 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (117822590..117860459)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (117095851..117133720)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Genetic tuning of beta-carotene oxygenase-1 activity rescues cone photoreceptor function in STRA6-deficient mice.
Title: Genetic tuning of β-carotene oxygenase-1 activity rescues cone photoreceptor function in STRA6-deficient mice.
beta-Carotene Oxygenase 1 Activity Modulates Circulating Cholesterol Concentrations in Mice and Humans.
Title: β-Carotene Oxygenase 1 Activity Modulates Circulating Cholesterol Concentrations in Mice and Humans.
provided novel experimental evidence that BCO1 and BCO2 could play a significant role in maintaining normal hepatic lipid and cholesterol homeostasis, potentially through the regulation of the FXR/miR-34a/SIRT1 pathway
Title: Ablation of carotenoid cleavage enzymes (BCO1 and BCO2) induced hepatic steatosis by altering the farnesoid X receptor/miR-34a/sirtuin 1 pathway.
This is the first demonstration that Bco1 disruption lowers murine circulating testosterone levels and thereby reduces prostatic androgen receptor signaling and prostatic cellular proliferation, further supporting the role of this protein in processes more diverse than carotenoid cleavage.
Title: Mice lacking β-carotene-15,15'-dioxygenase exhibit reduced serum testosterone, prostatic androgen receptor signaling, and prostatic cellular proliferation.
tissue- and sex-specific effects of beta-carotene 15,15' oxygenase (BCO1) on retinoid and lipid metabolism
Title: Tissue- and sex-specific effects of β-carotene 15,15' oxygenase (BCO1) on retinoid and lipid metabolism in adult and developing mice.
BCO1 modulates heart metabolism and function, possibly by altering levels of cofactors required for the actions of nuclear hormone receptors
Title: Cardiac dysfunction in β-carotene-15,15'-dioxygenase-deficient mice is associated with altered retinoid and lipid metabolism.
Dietary wolfberry elevated the xanthophyll concentrations and enhanced expression of BCO2 and heat shock protein 60, activated AMPKalpha2, potentiated mitophagy and mitochondrial biogenesis and enhanced lipid oxidation and secretion in the liver of mice.
Title: Wolfberries potentiate mitophagy and enhance mitochondrial biogenesis leading to prevention of hepatic steatosis in obese mice: the role of AMP-activated protein kinase α2 subunit.
Pleiotropic actions of Carotene-15,15'-monooxygenase and carotene-9',10'-monooxygenase supporting a strong role of these proteins in regulating tissue carotenoid accumulation as well as tomato carotenoid-independent regulation of lipid metabolism.
Title: Genetic ablation of carotene oxygenases and consumption of lycopene or tomato powder diets modulate carotenoid and lipid metabolism in mice.
in addition to cleaving beta-carotene to generate retinoids, CMOI serves an additional function(s) in retinoid and lipid metabolism and point to its role in the formation of specific lipids, possibly for use in nervous system tissue.
Title: Loss of β-carotene 15,15'-oxygenase in developing mouse tissues alters esterification of retinol, cholesterol and diacylglycerols.
a stepwise cleavage by BCO2 and BCO1 with APO10ol as an intermediate could provide a mechanism to tailor asymmetric carotenoids such as beta-cryptoxanthin for vitamin A production.
Title: Two carotenoid oxygenases contribute to mammalian provitamin A metabolism.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (2) 1 citation

General gene information

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Markers

Bcmo1 (e-PCR), detects polymorphism
- UniSTS:547962

Bcmo1 (e-PCR), detects polymorphism
- UniSTS:547963

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables beta-carotene 15,15'-dioxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables beta-carotene 15,15'-dioxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables beta-carotene 15,15'-dioxygenase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables beta-carotene 15,15'-dioxygenase activity	ISO Inferred from Sequence Orthology more info
enables beta-carotene 15,15'-dioxygenase activity	ISS Inferred from Sequence or Structural Similarity more info
enables carotenoid dioxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables dioxygenase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within beta-carotene metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in carotene catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in carotene catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in carotene catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in carotene catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in retinal metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within retinal metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in retinal metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retinal metabolic process	ISO Inferred from Sequence Orthology more info
involved_in retinoid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in retinoid metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: beta,beta-carotene 15,15'-dioxygenase

Names: beta,beta-carotene 15,15'-monooxygenase; beta-carotene 15, 15'-dioxygenase 1; beta-carotene 15,15' dioxygenase; beta-carotene 15,15'-monooxygenase; beta-carotene cleavage enzyme; beta-carotene dioxygenase 1; carotenoid-15,15'-oxygenase

NP_001156500.1

EC 1.13.11.63

NP_067461.2

EC 1.13.11.63

XP_011246783.1

EC 1.13.11.63

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001163028.2 → NP_001156500.1 beta,beta-carotene 15,15'-dioxygenase isoform 2

See identical proteins and their annotated locations for NP_001156500.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region resulting in a frameshift, compared to variant 1. It encodes isoform 2 which has a shorter and distinct C-terminus, compared to isoform 1.

Source sequence(s)

AC108436, AC121116, AEKQ02009580

Consensus CDS

CCDS52679.1

UniProtKB/TrEMBL

E9Q321

Related

ENSMUSP00000134815.2, ENSMUST00000176860.2

Conserved Domains (1) summary

pfam03055
Location:11 → 471

RPE65; Retinal pigment epithelial membrane protein
NM_021486.4 → NP_067461.2 beta,beta-carotene 15,15'-dioxygenase isoform 1

See identical proteins and their annotated locations for NP_067461.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1).

Source sequence(s)

AC108436, AC121116, AEKQ02009580

Consensus CDS

CCDS22697.1

UniProtKB/Swiss-Prot

Q6K1L5, Q8C6N5, Q9ERN9, Q9JJS6

UniProtKB/TrEMBL

Q05AC0

Related

ENSMUSP00000034308.9, ENSMUST00000034308.16

Conserved Domains (1) summary

pfam03055
Location:10 → 519

RPE65; Retinal pigment epithelial membrane protein