UCE target enrichment of Mononeuron_duguetiae_CNIN3416 - SRA

SRX7055031: UCE target enrichment of Mononeuron_duguetiae_CNIN3416
1 ILLUMINA (NextSeq 500) run: 1.4M spots, 406.4M bases, 184.5Mb downloads

Design: Genomic DNA was extracted using the Spin Column Genomic DNA Minipreps Kit (Bio Basic). Digestion of protein components was carried out overnight in 300 L of digestion solution and 20 L of proteinase K, and the supernatant was subsequently processed following the manufacturers protocol. All DNA extractions were quantified using a Qubit 2.0 (Life Technologies) and for each sample up to 50 ng of extracted DNA was fragmented to a mean fragment size of 400-600 bp using either a BioRuptor Pico sonication device or a Qsonica Q800R sonicator. Libraries were generated using Kapa Hyper Prep kits (Kapa Biosystems Inc., Wilmington, MA) and custom, TruSeq-style dual-indexing barcodes. For enrichment, we pooled libraries at equimolar concentrations and used the two Mybaits RNA bait libraries developed for Hymenoptera and synthesized by Arbor Biosciences (formerly MYcroarray, Ann Arbor, MI) following the standard enrichment protocol for UCEs (available from ultraconserved.org). The final enriched library pool quality was verified using an Agilent TapeSation 2200 (Agilent Tech). A first enriched pool was sent for sequencing to the University of Utah Genomics Core Facility on an Illumina HiSeq 2500 instrument (PE125, v4 chemistry), and a second to the Georgia Genomics Facility, University of Georgia, Athens, USA, on an Illumina NextSeq v2 300 cycle kit. Sequenced libraries produced 125 and 150 nucleotide paired-end reads respectively.

Submitted by: Instituto de Biologia, UNAM

Study: Phylogenomics and mitochondrial genome evolution of the gall-associated doryctine wasp genera (Hymenoptera: Braconidae)

PRJNA578939 • SRP226960 • All experiments • All runs

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In this project we characterized the mitogenomes of 13 representative species of the gall-associated doryctine clade (Hymenoptera: braconidae) and performed phylogenomic analyses with both mitogenome and ultraconserved element (UCE) DNA sequence data to assess the classification of Doryctinae and to examine the evolution of phytophagy within the group.

Sample:

SAMN13088731 • SRS5571018 • All experiments • All runs

Organism: Mononeuron duguetiae

Library:

Name: Mononeuron_duguetiae_CNIN3416

Instrument: NextSeq 500

Strategy: WGS

Source: GENOMIC

Selection: Hybrid Selection

Layout: PAIRED

Runs: 1 run, 1.4M spots, 406.4M bases, 184.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR10345151	1,357,036	406.4M	184.5Mb	2019-10-25

ID:: 9274101

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