Whole-genome sequencing of Enterobacteriaceae bacterium RIT702 - SRA

SRX7201388: Whole-genome sequencing of Enterobacteriaceae bacterium RIT702
1 ILLUMINA (Illumina MiSeq) run: 1.2M spots, 581.5M bases, 250.3Mb downloads

Design: RIT702 Was isolated by placing a wild mushroom fruiting body in LB broth. Genomic DNA from single colonies, plated onto the corresponding agar plates, was isolated using the QIAGEN DNeasy UltraClean Microbial Kit. Genomic DNA was quantified using a Qubit 3.0 fluorometer, and the genomic DNA was processed using the Nextera XT (Illumina) library preparation kit for sequencing using the MiSeq

Submitted by: Rochester Institute of Technology

Study: Isolation and Identification of Mushroom-Associated Bacteria from Black Creek Park (North Chili, NY)

PRJNA588411 • SRP231279 • All experiments • All runs

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The purpose of this study was to identify endophytic bacteria from wild mushrooms in a park near Rochester, NY, USA. Wild mushrooms were homogenized, and bacteria from the homogenate were isolated on agar plates. Single colonies were subcultured, and genomic DNA from single colonies was extracted and subjected to library preparation and whole-genome sequencing on an Illumina MiSeq.

Sample:

SAMN13243131 • SRS5706807 • All experiments • All runs

Organism: Enterobacteriaceae bacterium RIT702

Library:

Name: RIT702

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 1.2M spots, 581.5M bases, 250.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR10513277	1,223,830	581.5M	250.3Mb	2019-11-23

ID:: 9457645

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