TBR1509:mini-exon-seq - SRA

SRX7405170: TBR1509:mini-exon-seq
1 ILLUMINA (Illumina MiSeq) run: 26,920 spots, 16.2M bases, 10Mb downloads

Design: 1) Mini-exon PCR following Fernandes et al. 2001 (Trans R Soc Trop Med Hyg), 2) Gel-based purification (PureLink Quick Gel Extraction Kit, Thermo Fisher Scientific), 3) Index PCR, 4) Bead-based purification (Agencourt AMPure XP, Beckman Coulter), 5) Equimolar pooling, 6) 600-cycle Illumina MiSeq (Reagent Kit v3)

Submitted by: Pontifical Catholic University of Ecuador

Study: Trypanosoma cruzi and T. rangeli mini-exon sequencing

PRJNA596271 • SRP238060 • All experiments • All runs

show Abstracthide Abstract

Mini-exon fragments were amplified from T. cruzi and/or T. rangeli infected triatomine intestinal content by polymerase chain reaction and sequenced on the Illumina MiSeq platform. Triatomines (Rhodnius ecuadoriensis and Panstrongylus chinai) were collected in Loja Province, southern Ecuador. Sequencing also included amplicons generated from T. cruzi reference clone epimastigote DNA.

Sample:

SAMN13616552 • SRS5852748 • All experiments • All runs

Organism: Trypanosoma sp. TBR1509

Library:

Name: ME_TBR1509

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: METAGENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 26,920 spots, 16.2M bases, 10Mb

Run	# of Spots	# of Bases	Size	Published
SRR10729042	26,920	16.2M	10Mb	2020-05-01

ID:: 9691646

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