Genome sequencing and assembly of Aeromonas species - SRA

SRX7857854: Genome sequencing and assembly of Aeromonas species
1 ILLUMINA (Illumina HiSeq 4000) run: 3.3M spots, 996M bases, 394.1Mb downloads

Design: Genomic DNA was isolated from cultures grown in Lysogeny Broth overnight. DNA was extracted in 96-well format from 100 uL of sample using the MagAttract PowerMicrobiome DNA/RNA Kit (Qiagen, Hilden, Germany) automated on a Hamilton Microlab STAR robotic platform. Bead disruption was conducted on a TissueLyser II (20 Hz for 20 min) instrument in a 96 deep well plate in the presence of 200 uL phenol/chloroform. Genomic DNA was eluted in 90 ul water after magnetic bead cleanup. The resulting genomic DNA was quantified by Pico Green. The sequencing libraries were generated with the KAPA HyperPrep Kit. (Catalog number KK8504) and sequenced on the Illumina HiSeq4000 using a 150bpX2 paired end kit.

Submitted by: University of Maryland, School of Medicine

Study: Aeromonas Genome sequencing and assembly

PRJNA607550 • SRP251751 • All experiments • All runs

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Aeromonas species Genome sequencing and assembly

Sample:

SAMN14146685 • SRS6266955 • All experiments • All runs

Organism: Aeromonas sp. 600527

Library:

Name: 600527

Instrument: Illumina HiSeq 4000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 3.3M spots, 996M bases, 394.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR11247346	3,298,031	996M	394.1Mb	2021-04-01

ID:: 10283942

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