Whole genome sequencing of Avispirillum taxa - SRA

SRX16676228: Whole genome sequencing of Avispirillum taxa
1 ILLUMINA (Illumina MiSeq) run: 554,213 spots, 136.2M bases, 74Mb downloads

Design: Illumina libraries were prepared for all strains using the KAPA Low-Throughput Library Preparation Kit with Standard PCR Amplification Module (Kapa Biosystems, Wilmington, MA), following manufacturer's instructions except for the following changes: 750 ng DNA was sheared at 30 psi for 40 s and size selected to 700-770 bp following Illumina protocols. Standard desalting TruSeq LT and PCR Primers were ordered from Integrated DNA Technologies (Coralville, IA) and used at 0.375 and 0.5 M final concentrations, respectively. PCR was reduced to 3-5 cycles. Libraries were quantified using the KAPA Library Quantification Kit (Kapa), except with 10 l volume and 90-s annealing/extension PCR, then pooled and normalized to 4 nM. Pooled libraries were requantified by ddPCR on a QX200 system (Bio-Rad), using the Illumina TruSeq ddPCR Library Quantification Kit and following manufacturer's protocols, except with an extended 2-min annealing/extension time. The libraries were sequenced 2 250 bp paired end v2 on a MiSeq instrument (Illumina) at 13.5 pM, following manufacturer's protocols.

Submitted by: USDA, ARS, WRRC

Study: Campylobacter sp. RM12651 genome sequencing and assembly

PRJNA284972 • SRP388248 • All experiments • All runs

show Abstracthide Abstract

This genome is part of a larger study to sequence the genomes of all validly-described taxa within the genus Campylobacter

Sample:

SAMN14886848 • SRS14307764 • All experiments • All runs

Organism: Campylobacter sp. RM9331

Library:

Name: RM9331WGS_MiSeq

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 554,213 spots, 136.2M bases, 74Mb

Run	# of Spots	# of Bases	Size	Published
SRR20653228	554,213	136.2M	74Mb	2022-07-26

ID:: 23407919

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Sequence Read Archive

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