Raw reads of Cassiopea sp. - SRA

SRX21354309: Raw reads of Cassiopea sp.
1 ILLUMINA (Illumina HiSeq 4000) run: 314.6M spots, 95G bases, 32.4Gb downloads

Design: Genomic DNA was extracted using E.Z.N.A Mollusc DNA Kit (Omega Bio-tek) with a modified elution step. The first elute of 50 L was discarded to remove smaller fragments, and high quality gDNA was then retained and cleaned using Zymo Genomic DNA Clean and Concentrator. Sonication of purified gDNA to a mode fragment length of 200bp was done using Bioruptor Pico (Diagenode), and dual-indexed libraries were prepared using KAPA HyperPrep Kit (KK8502; KAPA Biosystems), according to manufacturer's recommendations, with a final double-size selection to narrow fragment size distribution prior to library amplification (six cycles). All cnidarian libraries were then pooled with six other samples of sponges in equimolar amounts and sequenced on a single HiSeq 4000 lane (150150bp).

Submitted by: National University of Singapore

Study: Revisiting mitogenome evolution in Medusozoa with eight new mitochondrial genomes

PRJNA934731 • SRP454847 • All experiments • All runs

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Seven new complete mitogenomes (two hydrozoans, five scyphozoans) and one new partial cubozoan mitogenome, sequenced using Illumina and assembled with SPAdes and NOVOPlasty

Sample: ZRC.CNI.3022

SAMN33774511 • SRS18599139 • All experiments • All runs

Organism: Cassiopea sp. ZRC CNI 3022

Library:

Name: ZRC.CNI.3022

Instrument: Illumina HiSeq 4000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 314.6M spots, 95G bases, 32.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR25627439	314,604,265	95G	32.4Gb	2023-08-14

ID:: 28791961

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