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SRX9044830: Target enrichment of UCEs and exons from Scytalopus bolivianus (Scyta_bolnus_L37419)
1 ILLUMINA (Illumina HiSeq 2000) run: 2.5M spots, 508.8M bases, 189.9Mb downloads

Design: We extracted whole genomic DNA from frozen tissues using standard methods and kits (Qiagen, Valencia, CA). Toepad DNA was extracted using a similar strategy, but in an ancient DNA lab where contamination risks were minimized (detailed protocol available at https://github.com/mgharvey/tyranni). We quantified DNA extracts using a Qubit fluorometer and sent them to Rapid Genomics (Gainesville, FL) for library preparation and sequence capture following the general protocol of Faircloth et al. 2012. This protocol involves fragmenting the genomic DNA, ligating barcoded Illumina-compatible adapters, PCR amplification, probe hybridization, and enrichment of hybridized fragments.
Submitted by: Louisiana State University
Study: Targeted enrichment of ultraconserved elements and exons from suboscine passerines
show Abstracthide Abstract
The tropics are the source of most biodiversity, yet inadequate sampling obscures answers to fundamental questions about how this diversity evolves. We leverage sampling assembled over decades of fieldwork to study diversification of the largest tropical bird radiation, suboscine passerines, by collecting sequence data using targeted enrichment of 2,389 genomic regions (2,321 UCEs and 96 exons) from 1,940 individuals of 1,287 species.
Sample:
SAMN15864589 • SRS7295090 • All experiments • All runs
Library:
Name: Scyta_bolnus_L37419
Instrument: Illumina HiSeq 2000
Strategy: WGS
Source: GENOMIC
Selection: Hybrid Selection
Layout: PAIRED
Runs: 1 run, 2.5M spots, 508.8M bases, 189.9Mb
Run# of Spots# of BasesSizePublished
SRR125559692,543,988508.8M189.9Mb2020-12-10

ID:
11751501

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