Name: HSKS6VI02
Instrument: 454 GS FLX Titanium
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: SINGLE
Construction protocol: Total RNA was isolated from spikes (about 3-6 cm in length) before heading, using an RNeasy Plant Mini Kit (QIAGEN, Hilden, Germany). mRNA was isolated from 48 ?g of RNA using an mRNA Purification Kit (Takara-Bio, Ohtsu, Japan). A 200 ng aliquot of mRNA was used to fragment the mRNA and synthesize cDNA from it using a cDNA Synthesis System (Roche Diagnostics, Mannheim, Germany). Approximately 1000,000,000 adapter-ligated cDNA molecules from the samples were used for library preparation using a GS FLX Titanium Rapid Library Preparation Kit (Roche Diagnostics).