mRNA sequencing of female 3rd instar larvae without imaginal discs of... - SRA

DRX049864: mRNA sequencing of female 3rd instar larvae without imaginal discs of Drosophila miranda (replicate 1)
1 ILLUMINA (Illumina HiSeq 2000) run: 91.5M spots, 18.5G bases, 12.4Gb downloads

Submitted by: National Institute of Genetics (Japan) (NIG)

Study: mRNA-seq of Drosophila miranda, D. pseudoobscura, and D. obscura

PRJDB4576 • DRP003328 • All experiments • All runs

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mRNA sequencing (mRNA-seq) was conducted in the three closely-related Drosophila species, i.e., D. miranda, D. pseudoobscura, and D. obscura to compare the transcriptomes and gene expression level.

Sample:

SAMD00047160 • DRS034843 • All experiments • All runs

Organism: Drosophila miranda

Library:

Name: mir17LdF1

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Construction protocol: Total RNA was extracted from 5 female 3rd instar larvae of D. miranda after removing imaginal discs but containing genital imaginal disc. mRNA was then purified by using oligo dT beads. Strand specific library was constructed with NEB #E7420. Sexing was based on the visual identification under microscopy and further confirmed by PCR with Y chromosome specific primers after the extraction of genomic DNA from same individuals.

Spot descriptor:

1 forward102 reverse

Runs: 1 run, 91.5M spots, 18.5G bases, 12.4Gb

Run	# of Spots	# of Bases	Size	Published
DRR055180	91,471,126	18.5G	12.4Gb	2016-11-08

ID:: 3411276

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