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DRX187898: Illumina MiSeq paired end sequencing of SAMD00192741
1 ILLUMINA (Illumina MiSeq) run: 6.3M spots, 3.6G bases, 2Gb downloads

Submitted by: KOBE_U
Study: Introgression of chromosomal segments conferring early heading time from wheat diploid progenitor, Aegilops tauschii Coss., into Japanese elite wheat cultivars
show Abstracthide Abstract
We backcrossed two early heading lines of synthetic hexaploid wheat with four Japanese elite cultivars to develop the early heading lines of bread wheat. In total, nine early heading lines, which showed two to eight days earlier heading time than their parental cultivars in the field conditions, were selected and established from the selfed progenies of the two- or three-times backcrossed populations. RNA sequencing-based genotyping was performed to detect single nucleotide polymorphisms between the selected lines and their parental wheat cultivars, which revealed the chromosomal regions transmitted from the parental synthetic wheat to the selected lines.
Sample: Seedling leaf of a synthetic wheat-derived line backcrossed with a bread cultivar SB12 No.1
SAMD00192741 • DRS125983 • All experiments • All runs
Library:
Name: haruyokoiSB12-1
Instrument: Illumina MiSeq
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: Oligo-dT
Layout: PAIRED
Construction protocol: TruSeq RNA Library Preparation kit v2
Spot descriptor:
forward302  reverse

Runs: 1 run, 6.3M spots, 3.6G bases, 2Gb
Run# of Spots# of BasesSizePublished
DRR1974816,315,9443.6G2Gb2020-03-09

ID:
10309450

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