SRA

Peripheral white blood cells (PWBCs) were obtained from 20 related domestic cats from two families and five unrelated cats (A6, A116, A165, A176 and A214). Each family was composed of ten cats representing three generations that were considered suitable for investigating the FLA allele and haplotype segregation. We first used the five unrelated cats to elucidate the alleles and specificity of expressed FLA-I loci in PWBCs and then used the 20 related cats for the NGS-based genotyping of expressible FLA-I and FLA-DRB loci. We have identified and presented novel alleles for the FLA-I and FLA-DRB genes and provided some further insights into the development of the NGS-based genotyping method at the RNA expression level, phylogenetic relationships and haplotype structures of FLA-I and FLA-DRB alleles and haplotypes in 20 cats from two families. This FLA polymorphism information could be useful as a standard internal control to establish the feline MHC genetic background for the benefit of biomedical research into disease associations such as infectious disease and autoimmune diseases in veterinary medical field.