PacBio RS II sequencing - SRA

ERX2413874: PacBio RS II sequencing
1 PACBIO_SMRT (PacBio RS II) run: 210,576 spots, 229.9M bases, 56.3Mb downloads

Design: RNA was extracted from an adult female broiler chicken spleen. 5' cap selection (Teloprime) and normalization (column) was performed on the library. The resulting cDNA was size selected for 0-2kb, 2-4kb, and >4kb parts. Each part was then prepared for Pacbio Iso-Seq sequencing. These were sequenced on an RSII machine. The resulting raw data was converted into bam format then converted into CCS reads using default parameters of the SMRT Package Iso-Seq pipeline. The CCS reads were then filtered for full length non-chimeric reads using the classify module of the Iso-Seq pipeline. The fastq file submitted represents the results from the classify output.

Submitted by: Roslin Institute, University of Edinburgh

Study: Iso-Seq with 5' cap selection and normalization on adult chicken spleen tissue.

PRJEB25379 • ERP107290 • All experiments • All runs

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RNA was extracted from an adult female broiler chicken spleen. 5' cap selection (Teloprime) and normalization (column) was performed on the library. The resulting cDNA was size selected for 0-2kb, 2-4kb, and >4kb parts. Each part was then prepared for Pacbio Iso-Seq sequencing. These were sequenced on an RSII machine. The resulting raw data was converted into bam format then converted into CCS reads using default parameters of the SMRT Package Iso-Seq pipeline. The CCS reads were then filtered for full length non-chimeric reads using the classify module of the Iso-Seq pipeline. The fastq file submitted represents the results from the classify output.

Sample: Chicken_Iso-Seq_Spleen

SAMEA104667333 • ERS2268716 • All experiments • All runs

Organism: Gallus gallus

Library:

Name: Chicken_Iso-Seq_Spleen1

Instrument: PacBio RS II

Strategy: OTHER

Source: TRANSCRIPTOMIC

Selection: Oligo-dT

Layout: SINGLE

Construction protocol: RNA was extracted from an adult female broiler chicken spleen. 5' cap selection (Teloprime) and normalization (column) was performed on the library. The resulting cDNA was size selected for 0-2kb, 2-4kb, and >4kb parts. Each part was then prepared for Pacbio Iso-Seq sequencing. These were sequenced on an RSII machine. The resulting raw data was converted into bam format then converted into CCS reads using default parameters of the SMRT Package Iso-Seq pipeline. The CCS reads were then filtered for full length non-chimeric reads using the classify module of the Iso-Seq pipeline. The fastq file submitted represents the results from the classify output.

Runs: 1 run, 210,576 spots, 229.9M bases, 56.3Mb

Run	# of Spots	# of Bases	Size	Published
ERR2365563	210,576	229.9M	56.3Mb	2018-03-30

ID:: 5313147

SRA

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