Name: 35_dpf_Male
Instrument: 454 GS FLX Titanium
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Gonads were sampled in 35 days post fertilization (dpf) embryos obtained from genetically all-male (XY) rainbow trout populations. RNA extraction was carried out using the MELT Total Nucleic Acid Isolation System (Applied Biosystems/Ambion). After cDNA synthesis and amplification using SMARTer PCR cDNA Synthesis (Takara Bio Europe/Clontech), cDNA was nebulisated and libraries were prepared using the GS DNA Library Preparation Kit according to manufacturer's recommendations (Roche Applied Science, Meylan, France). Final libraries were quantified with the SlingShot kit using the Fluidigm Digital Array and sequenced using the 454/Roche GS FLX Titanium chemistry (GS Titanium LV emPCR and sequencing kits) according to manufacturer's informations. Each sample was sequenced on one region of a two regions Picotiterplate.