Illumina HiSeq 2500 paired end sequencing; Use of the synergist piper... - SRA

ERX1409490: Illumina HiSeq 2500 paired end sequencing; Use of the synergist piperonyl butoxide slows the development of insecticide resistance in the whitefly Bemisia tabaci
3 ILLUMINA (Illumina HiSeq 2500) runs: 49.4M spots, 10G bases, 6.2Gb downloads

Design: Use of the synergist piperonyl butoxide slows the development of insecticide resistance in the whitefly Bemisia tabaci

Submitted by: College of Life and Environmental Sciences Penryn Campus, Cornwall University of Exeter UK (College of Life and Environmental Sciences Penryn )

Study: Use of the synergist piperonyl butoxide slows the development of insecticide resistance in the whitefly Bemisia tabaci

PRJEB13160 • ERP014700 • All experiments • All runs

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In the current study we examined the speed of resistance development in the silverleaf whitefly, Bemisia tabaci, after selection with either a neonicotinoid (thiacloprid) or pyrethroid (alpha-cypermethrin) insecticide alone or in combination with PBO. We used one control sample with no selection, 2 samples for neonicotinoid (thiacloprid) and Pyrethroid treatment and 2 samples for neonicotinoid (thiacloprid) + PBO and Pyrethroid + PBO treatments. The findings of this study demonstrate that PBO used in combination with certain insecticides can suppress the development of resistance in a laboratory setting.

Sample: B4

SAMEA3907496 • ERS1094630 • All experiments • All runs

Organism: Bemisia tabaci

Library:

Name: B4

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Construction protocol: Collection of adult specimens Pesticides treated plants were used for selection Column based extraction kit: Bioline Isolate RNA mini Kit Total RNA was used as a template for the generation of barcoded libraries (TrueSeq RNA library preparation, Illumina).

Spot descriptor:

1 forward102 reverse

Experiment attributes:

Experimental Factor: Thiacloprid + Piperonyl Butoxide selected: compound

Runs: 3 runs, 49.4M spots, 10G bases, 6.2Gb

Run	# of Spots	# of Bases	Size	Published
ERR1337906	16,492,985	3.3G	2.1Gb	2016-05-03
ERR1337907	15,888,854	3.2G	2Gb	2016-05-03
ERR1337908	17,048,620	3.4G	2.1Gb	2016-05-03

ID:: 2495473

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