Illumina HiSeq 2500 paired end sequencing; RNA-seq of different Bomby... - SRA

ERX2077817: Illumina HiSeq 2500 paired end sequencing; RNA-seq of different Bombyx mori tissues for males and females
1 ILLUMINA (Illumina HiSeq 2500) run: 28.2M spots, 7G bases, 2.7Gb downloads

Design: RNA-seq of different Bombyx mori tissues for males and females

Submitted by: IST Austria

Study: RNA-seq of different Bombyx mori tissues for males and females

PRJEB21541 • ERP023802 • All experiments • All runs

show Abstracthide Abstract

We sequenced mRNA of adult Bombyx mori males and females at 1 day after eclosion for the tissues/composite structures head, thorax, and gonads. The purpose of the study was to compare overall gene expression patterns of the Z chromosome and the autosomes between males and females in order to assess the status of dosage compensation.

Sample: Sample 10

SAMEA104142609 • ERS1801627 • All experiments • All runs

Organism: Bombyx mori

Library:

Name: Sample 10_p

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Construction protocol: Larvae were hatched in the lab at room temperature (~24°C) and fed at libitum with powdered mulberry leaves, reconstituted with water. RNA extractions were performed using the Qiagen RNeasy kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. Library preparation and sequencing were performed by the VBCF NGS Unit (www.vbcf.ac.at, Vienna, Austria).

Spot descriptor:

1 forward126 reverse

Experiment attributes:

Experimental Factor: organism part: thoracic segment of trunk

Experimental Factor: sex: female

Runs: 1 run, 28.2M spots, 7G bases, 2.7Gb

Run	# of Spots	# of Bases	Size	Published
ERR2018565	28,195,182	7G	2.7Gb	2017-07-19

ID:: 4290359

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