Name: 322Q
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: Oligo-dT
Layout: PAIRED
Construction protocol: 500 ng of total RNA were used as the input material and enriched for the m RNA fraction using oligo-dT magnetic beads. The mRNA was fragmented in the presence of divalent metal cations and at high temperature (resulting RNA fragment size was 80- 250 nt, with the major peak at 130nt). After first and second strand cDNA synthesis, the double stranded cDNA was end- repaired, 3 ́adenylated, and thereafter ligated to the Illumina barcoded adapters. After ligation, the product was enriched by 15 cycles of PCR.