Illumina HiSeq 2000 paired end sequencing; RNA-seq across tissues in ... - SRA

ERX2603463: Illumina HiSeq 2000 paired end sequencing; RNA-seq across tissues in four Notothenioid species (Antarctic icefish)
1 ILLUMINA (Illumina HiSeq 2000) run: 13.8M spots, 2.8G bases, 1.6Gb downloads

Design: RNA-seq across tissues in four Notothenioid species (Antarctic icefish)

Submitted by: Institut de biologie de lâ€™Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France (Institut de biologie de lâ€™Ecole normale superieu)

Study: RNA-seq across tissues in four Notothenioid species (Antarctic icefish)

PRJEB26835 • ERP108862 • All experiments • All runs

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We sequenced and assembled de novo the coding transcriptomes in four species of Notothenioid fish: Neopagetopsis ionah (Jonah's ice fish), Pseudochaenichtys georgianus (South Georgia icefish), Harpagifer antarcticus (Antarctic spiny plunderfish) and Parachaenichthys charcoti (Charcot's dragonfish). We sampled 1-4 individuals and 1-14 tissues (brain, white muscle, liver, head kidney, trunk kidney, skin, heart, red muscle, spleen, ovary, testis, whole blood, gill, red blood cells) in each species, depending on tissue availability.

Sample: Sample 50

SAMEA4669923 • ERS2490536 • All experiments • All runs

Organism: Pseudochaenichthys georgianus

Library:

Name: Sample 50_p

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Construction protocol: Samples were flash-frozen in liquid nitrogen upon dissection of wild-caught specimens. Total RNA was extracted using TRIsure (Bioline) according to manufacturer's instructions and purified on RNeasy columns (Qiagen). Libraries were prepared using Illumina TruSeq RNA Library Prep Kit according to manufacturer's instructions.

Spot descriptor:

1 forward102 reverse

Experiment attributes:

Experimental Factor: organism: Pseudochaenichthys georgianus

Experimental Factor: organism part: brain

Experimental Factor: cell type: not applicable

Runs: 1 run, 13.8M spots, 2.8G bases, 1.6Gb

Run	# of Spots	# of Bases	Size	Published
ERR2587187	13,818,468	2.8G	1.6Gb	2018-07-03

ID:: 5854832

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