Illumina HiSeq 2000 paired end sequencing; Time series RNAseq of apic... - SRA

ERX2613583: Illumina HiSeq 2000 paired end sequencing; Time series RNAseq of apical tissues along 4 developmental stages of development (I-III : vegetative stage; IV: sexually mature) in Mercurialis annua
1 ILLUMINA (Illumina HiSeq 2000) run: 26.5M spots, 5.3G bases, 3.1Gb downloads

Design: Time series RNAseq of apical tissues along 4 developmental stages of development (I-III : vegetative stage; IV: sexually mature) in Mercurialis annua

Submitted by: Department of Ecology and Evolution University of Lausanne (Department of Ecology and Evolution University of )

Study: Time series RNAseq of apical tissues along 4 developmental stages of development (I-III : vegetative stage; IV: sexually mature) in Mercurialis annua

PRJEB26957 • ERP108989 • All experiments • All runs

show Abstracthide Abstract

Sex-biased gene expression investigation in shared vegetative tissues from early on in development until sexual maturity in plants

Sample: IIF1

SAMEA4688816 • ERS2509001 • All experiments • All runs

Organism: Mercurialis annua

Library:

Name: IIF1_p

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Construction protocol: Cut of apical tissues, including early developping leaves and apical meristems, directly put in liquid nitrogen. Stages I : 1 week after germination (production of the first whorl of leaves); Stages II : 2 weeks after germination (second whorl of leaves); Stages III : 3 weeks after germination (third whorl of leaves); Stages IV : 6 weeks after germination (sexually mature individual) Plant growth in soil with 2 g of slow-release fertilizer per litre of soil Pools of tissues from 10 individuals. Total RNA extraction using RNeasy miny kit (Qiagen) TruSeq Stranded mRNA Sample Prep Kit (Illumina)

Spot descriptor:

1 forward101 reverse

Experiment attributes:

Experimental Factor: sex: female

Experimental Factor: developmental stage: LP.02 two leaves visible stage

Runs: 1 run, 26.5M spots, 5.3G bases, 3.1Gb

Run	# of Spots	# of Bases	Size	Published
ERR2597298	26,509,254	5.3G	3.1Gb	2018-07-03

ID:: 5842219

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