Illumina HiSeq 2000 paired end sequencing; RNASeq of mature leaf and ... - SRA

ERX2613829: Illumina HiSeq 2000 paired end sequencing; RNASeq of mature leaf and root tissues, before and after flowering, in Mercurialis annua
1 ILLUMINA (Illumina HiSeq 2000) run: 21.8M spots, 4.3G bases, 1.5Gb downloads

Design: RNASeq of mature leaf and root tissues, before and after flowering, in Mercurialis annua

Submitted by: Department of Ecology and Evolution University of Lausanne (Department of Ecology and Evolution University of )

Study: RNASeq of mature leaf and root tissues, before and after flowering, in Mercurialis annua

PRJEB26963 • ERP108996 • All experiments • All runs

show Abstracthide Abstract

Identification of patterns of sex-biased expression in (shared) vegetative tissues before and after sexual maturity in Mercurialis annua

Sample: H32

SAMEA4689062 • ERS2509247 • All experiments • All runs

Organism: Mercurialis annua

Library:

Name: H32_p

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Construction protocol: Cut of leaves or roots, directly put in liquid nitrogen. Stages I : 1 week after germination (production of the first whorl of leaves); Stages II : 2 weeks after germination (second whorl of leaves); Stages III : 3 weeks after germination (third whorl of leaves); Stages IV : 6 weeks after germination (sexually mature individual) Hydropony on Seramis clay RNeasy miny kit (Qiagen) TruSeq Stranded mRNA Sample Prep Kit (Illumina)

Spot descriptor:

1 forward101 reverse

Experiment attributes:

Experimental Factor: sex: female

Experimental Factor: developmental stage: whole plant flowering stage

Experimental Factor: organism part: leaf

Runs: 1 run, 21.8M spots, 4.3G bases, 1.5Gb

Run	# of Spots	# of Bases	Size	Published
ERR2597548	21,757,307	4.3G	1.5Gb	2018-07-03

ID:: 5857343

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