Illumina NovaSeq 6000 paired end sequencing; RNA-seq of healthy brain... - SRA

ERX3439278: Illumina NovaSeq 6000 paired end sequencing; RNA-seq of healthy brain, liver and muscle in 5 mammals
1 ILLUMINA (Illumina NovaSeq 6000) run: 196.5M spots, 58.9G bases, 16.5Gb downloads

Design: RNA-seq of healthy brain, liver and muscle in 5 mammals

Submitted by: European Molecular Biology Laboratory, European Bioinformatics Institute (European Molecular Biology Laboratory, European Bi)

Study: RNA-seq of healthy brain, liver and muscle in 5 mammals

PRJEB33353 • ERP116137 • All experiments • All runs

show Abstracthide Abstract

RNA-seq of total RNA was produced to aid in re-annotation of genes in horse, mouse, opossum, macaque, rat and pig. The RNA-seq was isolated from brain, liver and/or muscle.

Sample: do26153

SAMEA5756307 • ERS3559761 • All experiments • All runs

Organism: Rattus norvegicus

Library:

Name: do26153_p

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Inverse rRNA

Layout: PAIRED

Construction protocol: Primary tissue was flash-frozen with liquid nitrogen or dry ice within about 30 minutes post mortem, and stored at -80 C until RNA extraction. Frozen tissue was put into Trizol and homogenised with Precellys. RNA was then extracted with phenol::chloroform extraction and isopropanol precipitation. Illumina Stranded Total RNA Ribo-zero Gold

Experiment attributes:

Experimental Factor: organism: Rattus norvegicus

Experimental Factor: organism part: liver

Runs: 1 run, 196.5M spots, 58.9G bases, 16.5Gb

Run	# of Spots	# of Bases	Size	Published
ERR3415750	196,484,493	58.9G	16.5Gb	2019-09-03

ID:: 8948092

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