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ERX3566823: Illumina HiSeq 4000 paired end sequencing
1 ILLUMINA (Illumina HiSeq 4000) run: 111M spots, 16.6G bases, 5.9Gb downloads

Submitted by: Cambridge Department of Veterinary Medicine
Study: A high-quality Transcriptome Reference for the Tasmanian devil.
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This project provides a new transcriptome assembly for the Tasmanian devil (Sarcophilus harrisii). Frozen tissue RNA extractions were performed by Max Stammnitz (Department of Veterinary Medicine, University of Cambridge, UK).<br> <b>Data citation</b><br> For use of these resources, please cite: <b>The evolution of two transmissible cancers in Tasmanian devils (Stammnitz et al. 2023, Science 380:6642)</b> <b>Comment</b><br> RNA for this species transcriptome annotation was sourced from 33 Tasmanian devils between 2003 and 2017, and respectively sequenced on the equivalent of 1/3 of an Illumina HiSeq 4000 lane, yielding about 100 Million 75bp PE reads per sample. Mostly in biological duplicates, the featured tissues are: axillary nerve, bone marrow, brachial plexus nerve, brain stem, cerebellum, cerebrum, heart, liver, lung, optic nerve, ovary, pancreas, sciatic nerve, skin, spleen, testis, thyroid and trigeminal nerve.
Sample: testis.3
SAMEA5986015 • ERS3788837 • All experiments • All runs
Library:
Name: unspecified
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Qiagen Allprep extraction, KAPA rRNA depletion, random hexamer cDNA amplification, 75bp PE sequencing
Runs: 1 run, 111M spots, 16.6G bases, 5.9Gb
Run# of Spots# of BasesSizePublished
ERR3568424110,956,72016.6G5.9Gb2019-10-22

ID:
9243836

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