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ERX3717657: Illumina HiSeq 2000 paired end sequencing
1 ILLUMINA (Illumina HiSeq 2000) run: 5M spots, 748.2M bases, 305.5Mb downloads

Submitted by: UNIVERSITY OF OXFORD
Study: RNA-seq of Phlebotomus papatasi after feeding with blood, and blood containing Leishmania major, Leishmania donovani and Herpetomonas muscarum.
show Abstracthide Abstract
Leishmania are parasites that cause leishmaniasis, a compendium of serious diseases that affect millions of people, mainly across the subtropics and tropics. They are transmitted to humans by phlebotomine sand flies. However, despite establishment in the insect's midgut being key to transmission, early infection events inside the insect are still unclear. Here, we study the gene expression response of the insect vector to a Leishmania parasite that is able to establish infection (L. major) one that is unable to do so (L. donovani) as well as one that is not a natural parasite of sand flies (Herpetomonas muscarum). We found that responses following any of the infected blood meals was very similar to uninfected blood meal. However, changes post-blood meal from day 1 to day 9 were dramatic. As a blood feeding insect can accumulate five times its weight in one blood meal, this seems to be the most important physiological change rather than the presence of the parasite. The latter might be just one in a number of microbes the insect encounters. This will generate new thinking around the concept of stopping transmission by controlling the parasite inside the insect.
Sample: LdD4PF-3_1
SAMEA6369591 • ERS4135692 • All experiments • All runs
Library:
Name: unspecified
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: unspecified
Layout: PAIRED
Runs: 1 run, 5M spots, 748.2M bases, 305.5Mb
Run# of Spots# of BasesSizePublished
ERR37142104,988,219748.2M305.5Mb2020-05-01

ID:
10710292

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