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ERX779987: Illumina HiSeq 2500 paired end sequencing; Liver transcriptome profile associated with feed efficiency in Nellore Cattle (Bos indicus)
1 ILLUMINA (Illumina HiSeq 2500) run: 37.4M spots, 7.6G bases, 3Gb downloads

Design: Liver transcriptome profile associated with feed efficiency in Nellore Cattle (Bos indicus)
Submitted by: Faculty of Animal Science and Food Engineering - University of Sao Paulo (Faculty of Animal Science and Food Engineering - U)
Study: Liver transcriptome profile associated with feed efficiency in Nellore Cattle (Bos indicus)
show Abstracthide Abstract
8 animals of high feed efficiency and 8 animals of low feed efficiency, selected by the measure of residual intake and body weight gain at the end of a feeding trial, had their liver samples collected by biopsy and sequenced in a Illumina HiSeq2500 plataform
Sample: Sample 1
SAMEA3306049 • ERS684639 • All experiments • All runs
Organism: Bos indicus
Library:
Name: Sample 1
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: unspecified
Layout: PAIRED
Construction protocol: liver samples were collected by biopsy and, immediately frozen in liquid nitrogen and kept in -80°C freezer until RNA extraction RNeasy mini kit (QIAGEN, Crawley, West Sussex, UK) was used in accordance with guidelines supplied by manufacturer to extract total RNA from around 30 mg liver biopsy samples Preparation of libraries were carried out using TruSeq™ RNA Sample Prep Kit in accordance with TruSeq® RNA Sample Preparation v2 Guide (Illumina, USA, 2012, Part # 15026495 Rev. D)
Spot descriptor:
forward102  reverse

Experiment attributes:
Experimental Factor: diet: low feed efficiency
LIBRARY_STRAND: not applicable
Runs: 1 run, 37.4M spots, 7.6G bases, 3Gb
Run# of Spots# of BasesSizePublished
ERR78982237,446,5947.6G3Gb2015-03-18

ID:
1360255

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