Olea europaea transcriptome - SRA

SRX193576: Olea europaea transcriptome
8 LS454 (454 GS FLX Titanium) runs: 1.9M spots, 797.2M bases, 1.7Gb downloads

Design: Total RNA was purified from mesocarp samples following previously described methods (Castillejo et al. 2004, Hern*ndez et al, 2005) that allow elimination of phenolic compounds, and TURBO DNA-free kit (Ambion, USA) to remove genomic DNA. Oligotex (Quiagen) was used to obtain mRNA from total RNA to guarantee RNA integrity when mesocarp samples were to be used to generate cDNA libraries. Total RNA extraction from juvenile and adult roots, young leaves and stems, green and turning seeds, were carried out using Spectrum Total RNA Kit (Sigma-Aldrich, Spain) after tissue processing with an electric homogenizer. Total RNA from buds was purified using a Trizol-based method (Life Technologies) and treated twice with DNAase.23 In all cases, RNA concentration was quantified using a spectophotometer at OD260nm, and integrity checked by electrophoresis in agarose gels.

Submitted by: University of Malaga

Study: Olea europaea Transcriptome or Gene expression

PRJNA177329 • SRP016074 • All experiments • All runs

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Project aimed to get the olive transcriptome in different varieties of olive tree.

Sample: Olea europaea transcriptome

SAMN01761013 • SRS367899 • All experiments • All runs

Organism: Olea europaea

Library:

Instrument: 454 GS FLX Titanium

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PCR

Layout: SINGLE

Spot descriptor:

5 forward

Runs: 8 runs, 1.9M spots, 797.2M bases, 1.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR592583	272,857	135.2M	307.7Mb	2012-10-18
SRR596004	247,146	124.3M	282.5Mb	2012-10-19
SRR596243	273,519	147.5M	327.5Mb	2012-10-18
SRR596244	411,421	216.8M	486.6Mb	2012-10-18
SRR596246	171,762	40.5M	95.3Mb	2012-10-18
SRR596247	178,755	46.9M	96Mb	2012-10-18
SRR596269	79,473	25M	48.4Mb	2012-10-18
SRR596300	230,737	60.9M	122Mb	2012-10-18

ID:: 256397

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