RNA-seq of female larva Heterotrigona itama - SRA

SRX7158388: RNA-seq of female larva Heterotrigona itama
1 ILLUMINA (Illumina HiSeq 3000) run: 51.2M spots, 15.3G bases, 5.6Gb downloads

Design: After the QC procedures, mRNA from eukaryotic organisms is enriched from total RNA using oligo(dT) beads. The mRNA then fragmented randomly in fragmentation buffer, followed by cDNA synthesis using random hexamers and reverse transcriptase. After first-strand synthesis, a custom second-strand synthesis buffer (Illumina) is added, with dNTPs, RNase H and Escherichia coli polymerase I to generate the second strand by nick-translation and AMPure XP beads is used to purify the cDNA. The final cDNA library is ready after a round of purification, terminal repair, A-tailing, ligation of sequencing adapters, size selection and PCR enrichment. Library concentration was first quantified using a Qubit 2.0 fluorometer (Life Technologies), and then diluted to 1 ng/l before checking insert size on an Agilent 2100 and quantifying to greater accuracy by quantitative PCR (Q-PCR) (library activity >2 nM).

Submitted by: Malaysian Agricultural Research & Development Institute (MARDI)

Study: Transcriptome of Malaysian stingless bee (Heterotrigona itama)

PRJNA589983 • SRP230250 • All experiments • All runs

show Abstracthide Abstract

The study was conducted to sequence the transcripts purified from female stingless bee (Heterotrigona itama) at larval stage. Since the genome data of this particular species has not been sequenced (or made available for public view), transcriptome data is deemed feasible to analyze, much economical and important for 'entry point' functional genomics study.

Sample: Female larva pair-end 1

SAMN13301574 • SRS5665361 • All experiments • All runs

Organism: Heterotrigona itama

Library:

Name: EAS139

Instrument: Illumina HiSeq 3000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 51.2M spots, 15.3G bases, 5.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR10467123	51,150,231	15.3G	5.6Gb	2019-11-17

ID:: 9403655

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity