RNA-Seq of B. pyrosoma - SRA

SRX8742042: RNA-Seq of B. pyrosoma
1 ILLUMINA (Illumina HiSeq 2500) run: 82.2M spots, 24.7G bases, 7.5Gb downloads

Design: Each sample was separated to extract RNA using TRIzol Reagent (Life Technologies, USA). RNA degradation and contamination were monitored on 1% agarose gels. RNA purity was checked using the NanoPhotometer spectrophotometer (IMPLEN, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1.5 g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturers instructions.

Submitted by: Institute of Apicultural Research, Chinese Academy of Agricultural Sciences

Study: B. pyrosoma RNA-Seq

PRJNA646593 • SRP272166 • All experiments • All runs

show Abstracthide Abstract

comparative transcriptome reveal the adaptation mechanism of B. pyrosoma inhabiting on the Tibet Plateau

Sample:

SAMN15547572 • SRS7014241 • All experiments • All runs

Organism: Bombus pyrosoma

Library:

Name: Hg1

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq