PacBio Iso-Seq of Schistocerca gregaria: ovaries - SRA

SRX14134736: PacBio Iso-Seq of Schistocerca gregaria: ovaries
1 PACBIO_SMRT (Sequel II) run: 725,144 spots, 2.2G bases, 1.3Gb downloads

Design: RNA extraction was performed from dissected ovaries from a single female using Trizol and the Zymo Direct-zol 96 MagBead RNA kit. cDNA was synthesized from the RNA using the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification kit using the PacBio Iso-Seq Express Template Switching Oligo. The Iso-Seq library was prepared from cDNA with the PacBio Iso-Seq Express Library Preparation Using SMRTbell Express Template Prep Kit 2.0. Sequencing was performed on a Sequel II System using Binding Kit v2.0, Sequencing kit v2.0, and SMRT Cell 8M. To target HiFi reads, four libraries were barcoded individually and pooled for sequencing using a 30 hour movie time on one SMRTcell. Raw subreads were converted to HiFi data by processing with CCS to call a single high quality consensus sequence for each molecule, using a 99.5% consensus accuracy cutoff.

Submitted by: United States Department of Agriculture

Study: PacBio Iso-Seq transcriptome sequencing of Schistocerca gregaria tissues

PRJNA805283 • SRP359315 • All experiments • All runs

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PacBio Iso-Seq transcriptome sequencing of Schistocerca gregaria (desert locust) antennae, mouth parts (maxillary palpi, labial palpi, and labrum), brain, midgut, fat body, testes, and ovaries was conducted by the USDA-ARS Ag100Pest Initiative.

Sample: Schistocerca gregaria female ovaries

SAMN25851850 • SRS11960906 • All experiments • All runs

Organism: Schistocerca gregaria

Library:

Name: SGAP182_ovaries

Instrument: Sequel II

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Runs: 1 run, 725,144 spots, 2.2G bases, 1.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR17978908	725,144	2.2G	1.3Gb	2022-02-10

ID:: 19927155

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