show Abstracthide AbstractArachis duranensis has shown high tolerance to hydric stress. A 454 GS-FLX transcriptome analysis from cDNA collections from leaf and roots generated a total of 380,601 sequences which were assembled in 12,840 clusters. These data were used to sample large numbers of expressed genes and also to detect simple sequence repeats (SSRs) as these species are parentals for reference mapping populations. This study should facilitate basic and applied research on the genetics and evolutionary studies of peanut, contribute to the development of molecular markers for other legumes, and facilitate comparative genomics and the study of adaptive variation across the genus.