GSM5155630: EF 1; Bubalus bubalis; RNA-Seq - SRA

SRX10310790: GSM5155630: EF 1; Bubalus bubalis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 25.5M spots, 7.6G bases, 2.3Gb downloads

Submitted by: NCBI (GEO)

Study: Transcriptomics of granulosa cells(GCs) from healthy follicles(HFs), early atretic follicles(EFs) and late atretic follicle(LFs) in Chinese buffalo

PRJNA713391 • SRP310172 • All experiments • All runs

show Abstracthide Abstract

Compared with granulosa cells from healthy follicles, 2888 (1790 up-regulated, 1098 down-regulated) and 1629 (1230 up-regulated, 399 down-regulated) DEGs (|FC| > 1.75 and P value < 0.05) were identified in granulosa cells of early atretic follicles and late atretic follicle, respectively. Out of these DEGs, 1217 genes were commonly shared among the two comparisons Overall design: GCs were collected from healthy follicles, early atretic follicles and late atretic follicle for transcriptome analysis using second-generation high-throughput sequencing

Sample: EF 1

SAMN18250206 • SRS8431932 • All experiments • All runs

Organism: Bubalus bubalis

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Cells were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent(Invitrogen，Carlsbad，CA，USA). A total amount of 3 μg RNA per sample was used as an initial material for the RNA sample preparation. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM5155630

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 25.5M spots, 7.6G bases, 2.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR13931410	25,463,156	7.6G	2.3Gb	2021-03-18

ID:: 13438366

SRA

Sequence Read Archive

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