RNA seq of Athalia rosae: whole male L4 larvae - SRA

SRX10405233: RNA seq of Athalia rosae: whole male L4 larvae
1 ILLUMINA (Illumina NovaSeq 6000) run: 52.3M spots, 15.7G bases, 4.6Gb downloads

Design: Novogene (Cambridge, UK) carried out library preparation (with a Ribo-Zero for rRNA removal) see below for details from report:After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo(dT) beads, rRNA is removed using the Ribo-Zero kit that leaves the mRNA. The mRNA is then fragmented randomly by adding fragmentation buffer. The cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment. The quality control of library consists of three steps: 1) Qubit 2.0: tests the library concentration preliminarily, 2) Agilent 2100: tests the insert size, 3) Q-PCR: quantifies the library effective concentration precisely. The qualified libraries are fed into Illumina sequencers after pooling according to its effective concentration and expected data volume.

Submitted by: Univesitat Bielefeld

Study: Inter and intra-generational effects of larval starvation in Athalia rosae

PRJNA716060 • SRP311608 • All experiments • All runs

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We have shown that repeated bouts of starvation during early life have stronger intra-than inter-generational effects on life history traits and consumption measures in Athalia rosae.

Sample:

SAMN18393268 • SRS8527599 • All experiments • All runs

Organism: Athalia rosae

Library:

Name: Ar37

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 52.3M spots, 15.7G bases, 4.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR14028633	52,327,892	15.7G	4.6Gb	2022-04-30

ID:: 13723384

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