RNA-Seq of Lolium perenne: Ovary, Plant 79, Low transmission - SRA

SRX10993592: RNA-Seq of Lolium perenne: Ovary, Plant 79, Low transmission
1 ILLUMINA (Illumina HiSeq 4000) run: 86.1M spots, 17.2G bases, 5.6Gb downloads

Design: Samples (~ 3 mg) were powdered in liquid nitrogen using a micropestle in a 1.5 mL tube. The RNA extraction method used was a combination of an organic extraction from each sample using Trizol (Thermo Fisher Scientific, Waltham, USA) followed by further purification of 200 L of the Trizol/chloroform aqueous phase with an RNeasy MinElute Cleanup Kit (Qiagen, Valencia, USA). A TruSeq stranded mRNA sample preparation kit (Illumina, San Diego, CA, USA) was used to generate sequencing libraries from the total RNA extractions, and the indexed libraries were pooled and sequenced using the HiSeqTM 4000 platform by by BGI, Hong Kong.

Submitted by: AgResearch NZ Limited

Study: Plant disease resistance mechanisms are associated with the efficiency of Epichloe endophyte seed transmission in Lolium perenne

PRJNA732816 • SRP321377 • All experiments • All runs

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Endophyte transmission in perennial ryegrass is important for plant survival and commercial reasons but the underlying biological processes are poorly understood. We aim to understand the underlying biological processes of endophyte transmission in high- and low-transmitting perennial ryegrass by using transcriptomics and other methods.

Sample: Plant 79 Ovary

SAMN19341585 • SRS9067974 • All experiments • All runs

Organism: Lolium perenne

Library:

Name: OV_79

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 86.1M spots, 17.2G bases, 5.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR14655088	86,089,240	17.2G	5.6Gb	2022-03-03

ID:: 14621729

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