show Abstracthide AbstractThe domestic cat is an essential model for biomedical research as well as conservation of endangered felids. To expand our knowledge on processes happening in the testis during puberty, we performed RNA-seq on testicular tissues collected from adult and juvenile domestic cats. Additionally, we looked at the effect of vitrification protocol on testicular tissue transcriptome and compared the response in adult and juvenile samples, which allowed to gain new insights into tissue resilience.