Blood - SRA

SRX11333359: Blood
1 ILLUMINA (Illumina HiSeq 4000) run: 27M spots, 8.2G bases, 2.5Gb downloads

Design: The 3'end of eukaryotic mRNA has a structure of a polyA tail. Magnetic beads with Oligo (dT) are used to perform A-T base pairing with polyA, which can isolate mRNA from total RNA for analysis of transcriptome information. By adding fragmentation buffer, mRNA can be randomly fragmented, and small fragments of about 300 bp can be separated by magnetic bead screening. Under the action of reverse transcriptase, random hexamers were added to reverse synthesize cDNA using mRNA as a template. The double-stranded cDNA structure is a sticky end. Add End Repair Mix to make it a blunt end, and then add an "A" base to the 3'end to connect the Y-shaped linker. At last, the final cDNA libraries were sequenced on the Illumina platform.

Submitted by: Sichuan Agriculture University

Study: Transcriptome analysis of the effects of polysaccharide on Procambarus clarkii

PRJNA743233 • SRP326634 • All experiments • All runs

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Polysaccharides from plants have been an interesting subject in the biological activity against the infection by activating complement, regulating the production of cytokines, and inducing interferon. Procambarus clarkii, as one of the largest freshwater crustacean sources with high-quality protein and has significant commercial value. Therefor, it is of great significance to study the immune action of polysaccharide in crayfish.

Sample:

SAMN20003116 • SRS9375200 • All experiments • All runs

Organism: Procambarus clarkii

Library:

Name: A1

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Runs: 1 run, 27M spots, 8.2G bases, 2.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR15021497	26,998,725	8.2G	2.5Gb	2021-07-03

ID:: 15110182

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