Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Olfactory rosettes were obtained from each individual, flash frozen in liquid nitrogen and stored at -80 °C till RNA extraction. Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, USA) and analyzed with an Agilent Bioanalyzer 2100 total RNA Nano series II chip (Agilent). A transcriptome library for each individual was prepared from 2 microgram total RNA, using the Illumina TruSeq™ RNA Sample Prep Kit v2 following the manufacturer’s instructions (Illumina Inc.). RNA libraries were prepared for sequencing using standard Illumina protocols